Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-308-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Flt-3 Ligand/FLT3L Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Flt-3 Ligand/FLT3L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Flt-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain.
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Perier A, Gourier C, Pichard S, Husson J, Lajeunesse E, Babon A, Menez A, Gillet D
FEBS letters 2007 Nov 27;581(28):5480-4
FEBS letters 2007 Nov 27;581(28):5480-4
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Solanilla A, Déchanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
Blood 2000 Jun 15;95(12):3758-64
Blood 2000 Jun 15;95(12):3758-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Flt-3 Ligand and Neutral-ization by Human Flt-3 Ligand Antibody. Recombinant Human Flt-3 Ligand (Catalog # 308-FK) stimulates proliferation in BaFlt3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Flt-3 Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA). The ND50 is typically 0.02-0.06 µg/mL.