Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB10825 - Provider product page
- Provider
- Abnova Corporation
- Product name
- NEDD8 monoclonal antibody, clone 1A7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NEDD8.
- Isotype
- IgG
- Antibody clone number
- 1A7
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) against human NEDD8 (aa: 1-81) recombinant protein. (Expected MW is 40 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using NEDD8 monoclonal antibody, clone 1A7 (Cat # MAB10825) (green) and negative control (red).
- Validation comment
- Flow Cytometry
