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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10826 - Provider product page
- Provider
- Abnova Corporation
- Product name
- NEDD8 monoclonal antibody, clone 5B8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NEDD8.
- Isotype
- IgG
- Antibody clone number
- 5B8
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) against C6 (1) and HeLa (2) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) against human NEDD8 (aa: 1-81) recombinant protein. (Expected MW is 40 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using NEDD8 monoclonal antibody, clone 5B8 (Cat # MAB10826) (green) and negative control (red).
- Validation comment
- Flow Cytometry
