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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AM06718PU-N - Provider product page
- Provider
- OriGene
- Product name
- NEDD8 mouse monoclonal antibody, clone 1A7, Purified
- Antibody type
- Monoclonal
- Description
- NEDD8 mouse monoclonal antibody, clone 1A7, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NEDD8
- Isotype
- IgG
- Antibody clone number
- 1A7
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NEDD8 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red).
- Validation comment
- FC
