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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-72540 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRODH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRODH in mouse liver, kidney tissue using 35 µg of protein per lane. Samples were incubated with PRODH polyclonal antibody (Product # PA5-72540).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRODH in mouse liver, kidney tissue using 35 µg of protein per lane. Samples were incubated with PRODH polyclonal antibody (Product # PA5-72540).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PRODH in paraffin-embedded human skeletal muscle tissue. Samples were fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature. Antigen retrieval was performed by heat mediation with a citrate buffer (pH6). Samples were then incubated with PRODH polyclonal antibody (Product # PA5-72540) using a dilution of 1:25 for 1 hours at 37°C. An undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PRODH in A549 cells (green line). The cells were fixed with 2% paraformaldhyde (10 min) and permeabilized with 90% methanol (10 min). The sample was then incubated with 2% BSA followed by a PRODH polyclonal antibody (Product # PA5-72540) using a dilution of 1:25 for 60 min at 37°C. The secondary antibody used was goat-anti-rabbit IgG, DyLight 488 with a dilution of 1:200 for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 µg/1x10^6 cells) used under the same conditions with an acquisition of >10, 000 events.
