MA5-34776

antibody from Invitrogen Antibodies

Targeting: STUB1 CHIP, HSPABP2, NY-CO-7, SDCCAG7, UBOX1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34776

Provider

Invitrogen Antibodies

Product name

STUB1 Recombinant Rabbit Monoclonal Antibody (JG38-22)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: 293, MCF-7, SK-Br-3, mouse kidney tissue, 293T, LOVO, rat epididymis tissue, human colon cancer tissue, human placenta tissue, SH-SY-5Y.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG38-22

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of STUB1 in Lane 1: 293, Lane 2: MCF-7, Lane 3: SK-Br-3, Lane 4: Mouse kidney . Samples were incubated with STUB1 monoclonal antibody (Product # MA5-34776), at a dilution of 1:2000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of STUB1 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with STUB1 monoclonal antibody (Product # MA5-34776), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of STUB1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with STUB1 monoclonal antibody (Product # MA5-34776), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of STUB1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with STUB1 monoclonal antibody (Product # MA5-34776), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STUB1 in paraffin-embedded human placenta tissue. Samples were incubated with STUB1 monoclonal antibody (Product # MA5-34776), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STUB1 in paraffin-embedded rat epididymis tissue. Samples were incubated with STUB1 monoclonal antibody (Product # MA5-34776), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STUB1 in paraffin-embedded mouse kidney tissue. Samples were incubated with STUB1 monoclonal antibody (Product # MA5-34776), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STUB1 in paraffin-embedded human placenta tissue. Samples were incubated with STUB1 monoclonal antibody (Product # MA5-34776), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STUB1 in paraffin-embedded human colon cancer tissue. Samples were incubated with STUB1 monoclonal antibody (Product # MA5-34776), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of STUB1 in SH-SY-5Y cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with STUB1 monoclonal antibody (Product # MA5-34776) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.