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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NB100-77315 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77315, RRID:AB_1084262
- Product name
- Rabbit Polyclonal CHIP/STUB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references E3 ligase STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer disease patients.
Hsp70 and CHIP selectively mediate ubiquitination and degradation of hypoxia-inducible factor (HIF)-1alpha but Not HIF-2alpha.
Tsvetkov P, Adamovich Y, Elliott E, Shaul Y
The Journal of biological chemistry 2011 Mar 18;286(11):8839-45
The Journal of biological chemistry 2011 Mar 18;286(11):8839-45
Hsp70 and CHIP selectively mediate ubiquitination and degradation of hypoxia-inducible factor (HIF)-1alpha but Not HIF-2alpha.
Luo W, Zhong J, Chang R, Hu H, Pandey A, Semenza GL
The Journal of biological chemistry 2010 Feb 5;285(6):3651-63
The Journal of biological chemistry 2010 Feb 5;285(6):3651-63
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.
