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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP11581B - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP11581b, RRID:AB_10819137
- Product name
- SMYD4 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.41 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SMYD4 Antibody (C-term) (Cat. #AP11581b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the SMYD4 antibody detected the SMYD4 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SMYD4 Antibody (C-term) (Cat. #AP11581b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMYD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SMYD4 Antibody (C-term) (Cat. #AP11581b) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
