17594-1-AP

antibody from Invitrogen Antibodies

Targeting: SMYD4 KIAA1936, ZMYND21

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17594-1-AP

Provider

Invitrogen Antibodies

Product name

SMYD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KDSLTESFP AALAKTLEDA ALREENEQLS NASSSIGLCV DPLKGRCLVA TKDILPGELL VQEDAFVSVL NPGELPPPHH GLDSKWDTRV TNGDLYCHRC LKHTLATVPC DGCSYAKYCS QECLQQVWEL YHRTECPLGG LLLTLGVFCH IALRLTLLVG FEDVRKIITK LCDKISNKDI CLPESNNQVK TLNYGLGESE KNGNIVETPI PGCDINGKYE NNYNAVFNLL PHTENHSPEH KFLCALCVSA LCRQLEAASL QAIPTERIVN SSQLKAAVTP ELCPDVTIWG VAMLRHMLQL QCNAQAMTTI QHTGPKGSIV TDSRQVRLAT GIFPVISLLN HSCSPNTSVS FISTVATIRA SQRIRKGQEI LHCYGPHKS (201-578 aa encoded by BC035077)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 17594-1-AP (SMYD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17594-1-AP (SMYD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SMYD4 (IP:17594-1-AP, 4ug; Detection:17594-1-AP 1:500) with mouse brain tissue lysate 4000ug.