Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 135607 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#135607, RRID:AB_2566062
- Product name
- APC anti-mouse/human CD276 (B7-H3)
- Antibody type
- Monoclonal
- Antigen
- Mouse B7-H3 transfected L cell and P815
- Description
- B7-H3 is a type I transmembrane protein belonging to the B7 family of co-stimulatory proteins. B7-H3 is mostly expressed on professional APCs including B cells, macrophages, and dendritic cells at low levels. It is detected on various human and murine tumor cells, nasal and airway epithelial cells. Its expression on dendritic cells appears to be up-regulated by LPS. Initial studies have shown that B7-H3 provides a stimulatory signal to T cells. However, recent studies suggest a negative regulatory role for B7-H3 in T cell responses. Mouse B7-H3 protein inhibited T cell activation and effector cytokine production. Thus, the immunological function of B7-H3 remains unclear. B7-H3 is involved in the suppression of Th1-mediated immune responses and plays an important role in the development of pathogenic Th2 cells in a murine asthma model. Monoclonal antibody against B7-H3 enhances T cell proliferation in vitro and leads to exacerbated EAE in vivo. It has been reported that the Triggering Receptor Expressed on Myeloid cells (TREM)-like Transcript 2 (TLT-2, TREML2) is a receptor for B7-H3 in mice, although it remains controversial. Further studies are needed to identify the receptor of B7-H3.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Red dye
- Antibody clone number
- MIH35
- Vial size
- 25 µg
- Concentration
- 0.2 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Mouse B7-H3-transfected P815 cells were stained with CD276 (clone MIH35) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
- Conjugate
- Red dye