Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-88966 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88966, RRID:AB_11028981
- Product name
- Rabbit Polyclonal B7-H3/CD276 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human B7-H3/CD276 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Costimulatory protein 4IgB7H3 drives the malignant phenotype of glioblastoma by mediating immune escape and invasiveness.
Lemke D, Pfenning PN, Sahm F, Klein AC, Kempf T, Warnken U, Schnölzer M, Tudoran R, Weller M, Platten M, Wick W
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jan 1;18(1):105-17
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jan 1;18(1):105-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in human cell line RT-4 and human cell line U-251 MG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human placenta shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining in human placenta and pancreas tissues using anti-CD276 antibody. Corresponding CD276 RNA-seq data are presented for the same tissues.