MA5-15693

antibody from Invitrogen Antibodies

Targeting: CD276 B7-H3, B7H3, B7RP-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15693

Provider

Invitrogen Antibodies

Product name

B7-H3 Monoclonal Antibody (6A1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15693 targets CD276 in FACS, IHC, and WB applications and shows reactivity with Human samples.

Antibody clone number

6A1

Concentration

Conc. Not Determined

References

Submitted references

Humanized Affinity-matured Monoclonal Antibody 8H9 Has Potent Antitumor Activity and Binds to FG Loop of Tumor Antigen B7-H3.

Ahmed M, Cheng M, Zhao Q, Goldgur Y, Cheal SM, Guo HF, Larson SM, Cheung NK
The Journal of biological chemistry 2015 Dec 11;290(50):30018-29

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD276/B7H3 using CD276/B7H3 monoclonal antibody (Product # MA5-15693) in HEK293 (1) and CD276 (AA: 30-130) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 differentiated to Dendritic cells (Lane 2), LNCaP (Lane 3), U-2 OS (Lane 4), PC-3 (Lane 5), HeLa (Lane 6), MCF7 (Lane 7), Jurkat (Lane 8) and Raji (Lane 9). The blot was probed with Anti-B7-H3 Monoclonal Antibody (6A1) (Product # MA5-15693, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 100 kDa band corresponding to B7-H3 was detected in THP-1 differentiated to Dendritic cells, LNCaP, U-2 OS, PC-3, HeLa and MCF7 and not in Jurkat and Raji which are reported negative for B7-H3 expression.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276/B7H3 monoclonal antibody (Product # MA5-15693) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PC-3 cells using CD276/B7H3 monoclonal antibody (Product # MA5-15693) (green) and negative control (purple).