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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- GTX23705 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23705, RRID:AB_423840
- Product name
- Ap3D1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Chicken/Avian
- Host
- Goat
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 ?g of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.
