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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 600-101-290 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-101-290, RRID:AB_2056631
- Product name
- Anti-AP3D1 (GOAT) Antibody - 600-101-290
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Notch signaling from the endosome requires a conserved dileucine motif.
Zheng L, Saunders CA, Sorensen EB, Waxmonsky NC, Conner SD
Molecular biology of the cell 2013 Feb;24(3):297-307
Molecular biology of the cell 2013 Feb;24(3):297-307
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.
- Validation comment
- SDS PAGE
