NB100-1869AF488

antibody from Novus Biologicals

Targeting: SLC1A3 EA6, EAAT1, GLAST

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1869AF488

Provider

Novus Biologicals

Product name

Rabbit Polyclonal EAAT1/GLAST-1/SLC1A3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Green dye

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C in the dark.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor 488] [NB100-1869AF488] - Intracellular staining of HEK293 cells (1 x 10^6 cells/ml) with EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor® 488] [NB100-1869AF488] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.