MAB4354

antibody from R&D Systems

Targeting: CEACAM4 CGM7

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4354

Provider

R&D Systems

Product name

Human CEACAM-4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human CEACAM-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CEACAM-1, -3, -5, -6, -7, or -8 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

O75871


Isotype

IgG

Antibody clone number

568410

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CEACAM-4 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, IMR-90 human lung fibroblast cell line, NCI-H596 human lung adenosquamous carcinoma cell line, and human lung cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB4354) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEACAM-4 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CEACAM-4 in Human Lung Cancer Tissue. CEACAM-4 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB4354) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.