17-9818-41
antibody from Invitrogen Antibodies
Targeting: CLEC4C
BDCA2, CD303, CLECSF11, CLECSF7, DLEC, HECL
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 17-9818-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD303a Monoclonal Antibody (201A), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 201A monoclonal antibody reacts with human CD303a, which is also known as CLEC4C or Blood Dendritic Cell Antigen 2 (BDCA-2). This type II transmembrane protein is expressed almost exclusively on plasmacytoid dendritic cells, which secrete type I interferon during antiviral and antitumor responses. Studies have demonstrated that CD303a binds asialo-galactosyl-oligosaccharides on invading pathogens, resulting in the activation of signaling pathways that lead to CD303a internalization and downregulation of interferon production. Applications Reported: This 201A antibody has been reported for use in flow cytometric analysis. Applications Tested: This 201A antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 201A
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Impact of prior flavivirus immunity on Zika virus infection in rhesus macaques.
Cross-linking of CD81 by HCV-E2 protein inhibits human intrahepatic plasmacytoid dendritic cells response to CpG-ODN.
Human C-type lectin domain family 4, member C (CLEC4C/BDCA-2/CD303) is a receptor for asialo-galactosyl-oligosaccharides.
Isolation of human blood DC subtypes.
McCracken MK, Gromowski GD, Friberg HL, Lin X, Abbink P, De La Barrera R, Eckles KH, Garver LS, Boyd M, Jetton D, Barouch DH, Wise MC, Lewis BS, Currier JR, Modjarrad K, Milazzo M, Liu M, Mullins AB, Putnak JR, Michael NL, Jarman RG, Thomas SJ
PLoS pathogens 2017 Aug;13(8):e1006487
PLoS pathogens 2017 Aug;13(8):e1006487
Cross-linking of CD81 by HCV-E2 protein inhibits human intrahepatic plasmacytoid dendritic cells response to CpG-ODN.
Tu Z, Zhang P, Li H, Niu J, Jin X, Su L
Cellular immunology 2013 Jul-Aug;284(1-2):98-103
Cellular immunology 2013 Jul-Aug;284(1-2):98-103
Human C-type lectin domain family 4, member C (CLEC4C/BDCA-2/CD303) is a receptor for asialo-galactosyl-oligosaccharides.
Riboldi E, Daniele R, Parola C, Inforzato A, Arnold PL, Bosisio D, Fremont DH, Bastone A, Colonna M, Sozzani S
The Journal of biological chemistry 2011 Oct 14;286(41):35329-33
The Journal of biological chemistry 2011 Oct 14;286(41):35329-33
Isolation of human blood DC subtypes.
Kassianos AJ, Jongbloed SL, Hart DN, Radford KJ
Methods in molecular biology (Clifton, N.J.) 2010;595:45-54
Methods in molecular biology (Clifton, N.J.) 2010;595:45-54
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD123 PE (Product # 12-1239-42) and Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or Anti-Human CD303a APC (right). Cells in the monocyte and lymphocyte gate were used for analysis.