HPA037810

antibody from Atlas Antibodies

Targeting: TNNT3 AMCD2B, DA2B, DKFZp779M2348, FSSV

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA037810

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA037810, RRID:AB_10966217

Product name

Anti-TNNT3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RKPLNIDHLGEDKLRDKAKELWETLHQLEIDKFEF
GEKLKRQKYDITTLRSRIDQAQKHSKKAGTPAKGK
VG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human skeletal muscle and heart muscle tissues using Anti-TNNT3 antibody. Corresponding TNNT3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows low expression as expected.

Sample type

HUMAN