PA5-20631
antibody from Invitrogen Antibodies
Targeting: DISP2
C15orf36, DISPB, HsT16908, KIAA1742, LINC00594
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-20631 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DISP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat brain tissue lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain tissue lysate using a Disp2 polyclonal antibody (Product # PA5-20631) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Disp2 in rat brain tissue lysate with DISP2 Polyclonal Antibody (Product # PA5-20631) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DISP2 Polyclonal Antibody (Product # PA5-20631) and a 150kDa band corresponding to DISP2 was observed across cell lines and tissues tested. Membrane enriched cell extracts (30 µg lysate) of Neuro-2a (Lane 1), RAW 264.7 (Lane 2), Mouse Brain (Lane 3), Rat Brain (Lane 4) and Mouse Uterus (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2µg/ml dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..