TA500557

antibody from OriGene

Targeting: FKBP1A FKBP-12, FKBP1, FKBP12, FKBP12C, PKC12, PPIASE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500557

Provider

OriGene

Proper citation

OriGene Cat#TA500557, RRID:AB_11124838

Product name

FKBP1A (FKBP12) mouse monoclonal antibody, clone OTI3B3 (formerly 3B3)

Antibody type

Monoclonal

Description

FKBP1A (FKBP12) mouse monoclonal antibody, clone OTI3B3 (formerly 3B3)

Host

Mouse

Conjugate

Unconjugated

Epitope

FKBP1A

Isotype

IgG

Antibody clone number

OTI3B3

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP1A (RC201237, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP1A.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FKBP1A monoclonal antibody.(1:200)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FKBP1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500557)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FKBP1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500557)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC201237 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FKBP1A antibody(TA500557), and then analyzed by flow cytometry.

Validation comment

FC