Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 66417-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAP3 Monoclonal Antibody (1B11B8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: NANEPPLVF VGKGITFDSG GISIKASANM DLMRADMGGA ATICSAIVSA AKLNLPINII GLAPLCENMP SGKANKPGDV VRAKNGKTIQ VDNTDAEGRL ILADALCYAH TFNPKVILNA ATLTGAMDVA LGSGATGVFT NSSWLWNKLF EASIETGDRV WRMPLFEHYT RQVVDCQLAD VNNIGKYRSA GACTAAAFLK EFVTHPKWAH LDIAGVMTNK DEVPYLRKGM TGRPTRTLIE FLLRFSQDNA (271-519 aa encoded by BC065564)
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B11B8
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-IG ( LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C2C12 cell were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (Product # 66417-1-IG, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung tissue slide using 66417-1-IG ( LAP3 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung tissue slide using 66417-1-IG ( LAP3 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).