Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX631395 - Provider product page
- Provider
- GeneTex
- Product name
- ELP3 antibody [GT11811]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- ELP3 antibody detects ELP3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody (GTX631395) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELP3 antibody detects ELP3 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody (GTX631395) diluted by 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELP3 antibody [GT11811] detects ELP3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ELP3 protein stained by ELP3 antibody [GT11811] (GTX631395) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ELP3 protein from 293T whole cell extracts using 5 £gg of ELP3 antibody [GT11811] (GTX631395).Western blot analysis was performed using ELP3 antibody [GT11811] (GTX631395).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.