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- Antibody Data
- Antigen structure
- References [38]
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- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- ABIN151073 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Egl Nine Homolog 3 (C. Elegans) (EGLN3) antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 mL
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
- Handling
- Do not freeze.
Submitted references HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma.
Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1α stabilization.
NQO1 inhibits proteasome-mediated degradation of HIF-1α.
SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status.
Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult.
Distinct breast cancer stem/progenitor cell populations require either HIF1α or loss of PHD3 to expand under hypoxic conditions.
Prolyl-4-hydroxylase domain protein 2 controls NF-κB/p65 transactivation and enhances the catabolic effects of inflammatory cytokines on cells of the nucleus pulposus.
Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27.
Prolyl-4-hydroxylase 3 (PHD3) expression is downregulated during epithelial-to-mesenchymal transition.
Cross-talk between hypoxia and insulin signaling through Phd3 regulates hepatic glucose and lipid metabolism and ameliorates diabetes.
Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer.
Cellular overexpression of aquaporins slows down the natural HIF-2α degradation during prolonged hypoxia.
Expression of prolyl hydroxylases (PHDs) is selectively controlled by HIF-1 and HIF-2 proteins in nucleus pulposus cells of the intervertebral disc: distinct roles of PHD2 and PHD3 proteins in controlling HIF-1α activity in hypoxia.
The population growth consequences of variation in individual heterozygosity.
Prolyl hydroxylase PHD3 enhances the hypoxic survival and G1 to S transition of carcinoma cells.
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
Aberrant promoter CpG methylation is a mechanism for impaired PHD3 expression in a diverse set of malignant cells.
Regulation of cellular levels of Sprouty2 protein by prolyl hydroxylase domain and von Hippel-Lindau proteins.
Expression of the cellular oxygen sensor PHD2 (EGLN-1) predicts radiation sensitivity in squamous cell cancer of the head and neck.
HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury.
Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.
Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL.
Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation.
Glutamate receptor dynamics in dendritic microdomains.
PHDs overactivation during chronic hypoxia "desensitizes" HIFalpha and protects cells from necrosis.
mAKAP compartmentalizes oxygen-dependent control of HIF-1alpha.
Expression and actions of HIF prolyl-4-hydroxylase in the rat kidneys.
Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants.
Prolyl hydroxylase inhibitors delay neuronal cell death caused by trophic factor deprivation.
EGLN3 prolyl hydroxylase regulates skeletal muscle differentiation and myogenin protein stability.
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation.
An alternatively spliced transcript of the PHD3 gene retains prolyl hydroxylase activity.
Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness.
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Endogenous 2-oxoacids differentially regulate expression of oxygen sensors.
Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor.
A conserved family of prolyl-4-hydroxylases that modify HIF.
Miikkulainen P, Högel H, Rantanen K, Suomi T, Kouvonen P, Elo LL, Jaakkola PM
Cancer & metabolism 2017;5:5
Cancer & metabolism 2017;5:5
Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1α stabilization.
Bruno T, Valerio M, Casadei L, De Nicola F, Goeman F, Pallocca M, Catena V, Iezzi S, Sorino C, Desantis A, Manetti C, Blandino G, Floridi A, Fanciulli M
Journal of experimental & clinical cancer research : CR 2017 Feb 18;36(1):32
Journal of experimental & clinical cancer research : CR 2017 Feb 18;36(1):32
NQO1 inhibits proteasome-mediated degradation of HIF-1α.
Oh ET, Kim JW, Kim JM, Kim SJ, Lee JS, Hong SS, Goodwin J, Ruthenborg RJ, Jung MG, Lee HJ, Lee CH, Park ES, Kim C, Park HJ
Nature communications 2016 Dec 14;7:13593
Nature communications 2016 Dec 14;7:13593
SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status.
Adam MG, Matt S, Christian S, Hess-Stumpp H, Haegebarth A, Hofmann TG, Algire C
Cell cycle (Georgetown, Tex.) 2015;14(23):3734-47
Cell cycle (Georgetown, Tex.) 2015;14(23):3734-47
Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult.
Vogler M, Zieseniss A, Hesse AR, Levent E, Tiburcy M, Heinze E, Burzlaff N, Schley G, Eckardt KU, Willam C, Katschinski DM
Pflugers Archiv : European journal of physiology 2015 Oct;467(10):2141-9
Pflugers Archiv : European journal of physiology 2015 Oct;467(10):2141-9
Distinct breast cancer stem/progenitor cell populations require either HIF1α or loss of PHD3 to expand under hypoxic conditions.
Iriondo O, Rábano M, Domenici G, Carlevaris O, López-Ruiz JA, Zabalza I, Berra E, Vivanco Md
Oncotarget 2015 Oct 13;6(31):31721-39
Oncotarget 2015 Oct 13;6(31):31721-39
Prolyl-4-hydroxylase domain protein 2 controls NF-κB/p65 transactivation and enhances the catabolic effects of inflammatory cytokines on cells of the nucleus pulposus.
Li J, Yuan W, Jiang S, Ye W, Yang H, Shapiro IM, Risbud MV
The Journal of biological chemistry 2015 Mar 13;290(11):7195-207
The Journal of biological chemistry 2015 Mar 13;290(11):7195-207
Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27.
Högel H, Miikkulainen P, Bino L, Jaakkola PM
Molecular cancer 2015 Jul 30;14:143
Molecular cancer 2015 Jul 30;14:143
Prolyl-4-hydroxylase 3 (PHD3) expression is downregulated during epithelial-to-mesenchymal transition.
Place TL, Nauseef JT, Peterson MK, Henry MD, Mezhir JJ, Domann FE
PloS one 2013;8(12):e83021
PloS one 2013;8(12):e83021
Cross-talk between hypoxia and insulin signaling through Phd3 regulates hepatic glucose and lipid metabolism and ameliorates diabetes.
Taniguchi CM, Finger EC, Krieg AJ, Wu C, Diep AN, LaGory EL, Wei K, McGinnis LM, Yuan J, Kuo CJ, Giaccia AJ
Nature medicine 2013 Oct;19(10):1325-30
Nature medicine 2013 Oct;19(10):1325-30
Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer.
Rawluszko AA, Bujnicka KE, Horbacka K, Krokowicz P, Jagodziński PP
BMC cancer 2013 Nov 6;13:526
BMC cancer 2013 Nov 6;13:526
Cellular overexpression of aquaporins slows down the natural HIF-2α degradation during prolonged hypoxia.
Galán-Cobo A, Sánchez-Silva R, Serna A, Abreu-Rodríguez I, Muñoz-Cabello AM, Echevarría M
Gene 2013 Jun 10;522(1):18-26
Gene 2013 Jun 10;522(1):18-26
Expression of prolyl hydroxylases (PHDs) is selectively controlled by HIF-1 and HIF-2 proteins in nucleus pulposus cells of the intervertebral disc: distinct roles of PHD2 and PHD3 proteins in controlling HIF-1α activity in hypoxia.
Fujita N, Markova D, Anderson DG, Chiba K, Toyama Y, Shapiro IM, Risbud MV
The Journal of biological chemistry 2012 May 11;287(20):16975-86
The Journal of biological chemistry 2012 May 11;287(20):16975-86
The population growth consequences of variation in individual heterozygosity.
Di Fonzo MM, Pelletier F, Clutton-Brock TH, Pemberton JM, Coulson T
PloS one 2011;6(5):e19667
PloS one 2011;6(5):e19667
Prolyl hydroxylase PHD3 enhances the hypoxic survival and G1 to S transition of carcinoma cells.
Högel H, Rantanen K, Jokilehto T, Grenman R, Jaakkola PM
PloS one 2011;6(11):e27112
PloS one 2011;6(11):e27112
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL
Cell 2011 May 27;145(5):732-44
Cell 2011 May 27;145(5):732-44
Aberrant promoter CpG methylation is a mechanism for impaired PHD3 expression in a diverse set of malignant cells.
Place TL, Fitzgerald MP, Venkataraman S, Vorrink SU, Case AJ, Teoh ML, Domann FE
PloS one 2011 Jan 28;6(1):e14617
PloS one 2011 Jan 28;6(1):e14617
Regulation of cellular levels of Sprouty2 protein by prolyl hydroxylase domain and von Hippel-Lindau proteins.
Anderson K, Nordquist KA, Gao X, Hicks KC, Zhai B, Gygi SP, Patel TB
The Journal of biological chemistry 2011 Dec 9;286(49):42027-36
The Journal of biological chemistry 2011 Dec 9;286(49):42027-36
Expression of the cellular oxygen sensor PHD2 (EGLN-1) predicts radiation sensitivity in squamous cell cancer of the head and neck.
Luukkaa M, Jokilehto T, Kronqvist P, Vahlberg T, Grénman R, Jaakkola P, Minn H
International journal of radiation biology 2009;85(10):900-8
International journal of radiation biology 2009;85(10):900-8
HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury.
Schödel J, Klanke B, Weidemann A, Buchholz B, Bernhardt W, Bertog M, Amann K, Korbmacher C, Wiesener M, Warnecke C, Kurtz A, Eckardt KU, Willam C
The American journal of pathology 2009 May;174(5):1663-74
The American journal of pathology 2009 May;174(5):1663-74
Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.
Chan DA, Kawahara TL, Sutphin PD, Chang HY, Chi JT, Giaccia AJ
Cancer cell 2009 Jun 2;15(6):527-38
Cancer cell 2009 Jun 2;15(6):527-38
Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL.
Xie L, Xiao K, Whalen EJ, Forrester MT, Freeman RS, Fong G, Gygi SP, Lefkowitz RJ, Stamler JS
Science signaling 2009 Jul 7;2(78):ra33
Science signaling 2009 Jul 7;2(78):ra33
Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation.
Rantanen K, Pursiheimo J, Högel H, Himanen V, Metzen E, Jaakkola PM
Molecular biology of the cell 2008 May;19(5):2231-40
Molecular biology of the cell 2008 May;19(5):2231-40
Glutamate receptor dynamics in dendritic microdomains.
Newpher TM, Ehlers MD
Neuron 2008 May 22;58(4):472-97
Neuron 2008 May 22;58(4):472-97
PHDs overactivation during chronic hypoxia "desensitizes" HIFalpha and protects cells from necrosis.
Ginouvès A, Ilc K, Macías N, Pouysségur J, Berra E
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 25;105(12):4745-50
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 25;105(12):4745-50
mAKAP compartmentalizes oxygen-dependent control of HIF-1alpha.
Wong W, Goehring AS, Kapiloff MS, Langeberg LK, Scott JD
Science signaling 2008 Dec 23;1(51):ra18
Science signaling 2008 Dec 23;1(51):ra18
Expression and actions of HIF prolyl-4-hydroxylase in the rat kidneys.
Li N, Yi F, Sundy CM, Chen L, Hilliker ML, Donley DK, Muldoon DB, Li PL
American journal of physiology. Renal physiology 2007 Jan;292(1):F207-16
American journal of physiology. Renal physiology 2007 Jan;292(1):F207-16
Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants.
Bai JY, Zeng L, Hu YL, Li YF, Lin ZP, Shang SC, Shi YS
Biotechnology letters 2007 Dec;29(12):2007-12
Biotechnology letters 2007 Dec;29(12):2007-12
Prolyl hydroxylase inhibitors delay neuronal cell death caused by trophic factor deprivation.
Lomb DJ, Straub JA, Freeman RS
Journal of neurochemistry 2007 Dec;103(5):1897-906
Journal of neurochemistry 2007 Dec;103(5):1897-906
EGLN3 prolyl hydroxylase regulates skeletal muscle differentiation and myogenin protein stability.
Fu J, Menzies K, Freeman RS, Taubman MB
The Journal of biological chemistry 2007 Apr 27;282(17):12410-8
The Journal of biological chemistry 2007 Apr 27;282(17):12410-8
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
American journal of physiology. Lung cellular and molecular physiology 2006 Oct;291(4):L588-95
American journal of physiology. Lung cellular and molecular physiology 2006 Oct;291(4):L588-95
Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation.
Khan Z, Michalopoulos GK, Stolz DB
The American journal of pathology 2006 Oct;169(4):1251-69
The American journal of pathology 2006 Oct;169(4):1251-69
An alternatively spliced transcript of the PHD3 gene retains prolyl hydroxylase activity.
Cervera AM, Apostolova N, Luna-Crespo F, Sanjuan-Pla A, Garcia-Bou R, McCreath KJ
Cancer letters 2006 Feb 20;233(1):131-8
Cancer letters 2006 Feb 20;233(1):131-8
Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness.
Jokilehto T, Rantanen K, Luukkaa M, Heikkinen P, Grenman R, Minn H, Kronqvist P, Jaakkola PM
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Feb 15;12(4):1080-7
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Feb 15;12(4):1080-7
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Günzler V, White CW
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
Endogenous 2-oxoacids differentially regulate expression of oxygen sensors.
Dalgard CL, Lu H, Mohyeldin A, Verma A
The Biochemical journal 2004 Jun 1;380(Pt 2):419-24
The Biochemical journal 2004 Jun 1;380(Pt 2):419-24
Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor.
Ivan M, Haberberger T, Gervasi DC, Michelson KS, Günzler V, Kondo K, Yang H, Sorokina I, Conaway RC, Conaway JW, Kaelin WG Jr
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 15;99(21):13459-64
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 15;99(21):13459-64
A conserved family of prolyl-4-hydroxylases that modify HIF.
Bruick RK, McKnight SL
Science (New York, N.Y.) 2001 Nov 9;294(5545):1337-40
Science (New York, N.Y.) 2001 Nov 9;294(5545):1337-40
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