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- Antibody Data
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- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-1393 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1393, RRID:AB_2196645
- Product name
- Goat Polyclonal STK35 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein.
Vallenius T, Mäkelä TP
Journal of cell science 2002 May 15;115(Pt 10):2067-73
Journal of cell science 2002 May 15;115(Pt 10):2067-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK35 Antibody [NB100-1393] - Staining of Mouse with antibody at 1 ug/mL (A) and Pig with antibody at 2 ug/mL (B). Testes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK35 Antibody [NB100-1393] - Staining of nuclear HEK293 with antibody at 2 ug/mL (A) and Human Breast cancer with antibody at 1 ug/mL (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STK35 Antibody [NB100-1393] - Staining of Human Testis with antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: STK35 Antibody [NB100-1393] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
