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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB7918 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GCHFR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GCHFR in ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P30047- Isotype
- IgG
- Antibody clone number
- 846011
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse GCHFR by Western Blot. Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GCHFR by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
