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- Antibody Data
- Antigen structure
- References [21]
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- Product number
- MAB1859 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Dectin-1/CLEC7A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Dectin-1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_072092- Isotype
- IgG
- Antibody clone number
- 259931
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis.
Cellular metabolism constrains innate immune responses in early human ontogeny.
EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans.
Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses.
Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells.
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro.
TSLP production by dendritic cells is modulated by IL-1β and components of the endoplasmic reticulum stress response.
Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
Podosomes of dendritic cells facilitate antigen sampling.
Human intestinal epithelial cells respond to β-glucans via Dectin-1 and Syk.
The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum.
Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway.
MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.
Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome.
Protein kinase Cδ is a critical component of Dectin-1 signaling in primary human monocytes.
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2.
de Quaglia E Silva JC, Della Coletta AM, Gardizani TP, Romagnoli GG, Kaneno R, Dias-Melicio LA
Journal of immunology research 2019;2019:1529189
Journal of immunology research 2019;2019:1529189
Cellular metabolism constrains innate immune responses in early human ontogeny.
Kan B, Michalski C, Fu H, Au HHT, Lee K, Marchant EA, Cheng MF, Anderson-Baucum E, Aharoni-Simon M, Tilley P, Mirmira RG, Ross CJ, Luciani DS, Jan E, Lavoie PM
Nature communications 2018 Nov 16;9(1):4822
Nature communications 2018 Nov 16;9(1):4822
EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans.
Swidergall M, Solis NV, Lionakis MS, Filler SG
Nature microbiology 2018 Jan;3(1):53-61
Nature microbiology 2018 Jan;3(1):53-61
Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses.
Kaisar MMM, Ritter M, Del Fresno C, Jónasdóttir HS, van der Ham AJ, Pelgrom LR, Schramm G, Layland LE, Sancho D, Prazeres da Costa C, Giera M, Yazdanbakhsh M, Everts B
PLoS biology 2018 Apr;16(4):e2005504
PLoS biology 2018 Apr;16(4):e2005504
Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells.
Yeh CC, Horng HC, Chou H, Tai HY, Shen HD, Hsieh SL, Wang PH
International journal of molecular sciences 2017 Mar 13;18(3)
International journal of molecular sciences 2017 Mar 13;18(3)
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro.
Smith IM, Baker A, Christensen JE, Boekhout T, Frøkiær H, Arneborg N, Jespersen L
PloS one 2016;11(11):e0167410
PloS one 2016;11(11):e0167410
TSLP production by dendritic cells is modulated by IL-1β and components of the endoplasmic reticulum stress response.
Elder MJ, Webster SJ, Williams DL, Gaston JS, Goodall JC
European journal of immunology 2016 Feb;46(2):455-63
European journal of immunology 2016 Feb;46(2):455-63
Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
Gazendam RP, van Hamme JL, Tool AT, Hoogenboezem M, van den Berg JM, Prins JM, Vitkov L, van de Veerdonk FL, van den Berg TK, Roos D, Kuijpers TW
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1272-83
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1272-83
Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
Fernandes RK, Bachiega TF, Rodrigues DR, Golim Mde A, Dias-Melicio LA, Balderramas Hde A, Kaneno R, Soares ÂM
PloS one 2015;10(3):e0120948
PloS one 2015;10(3):e0120948
Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li XH, Hori T, Zurawski S, Liu YJ, Zurawski G, Oh S
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1723-31
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1723-31
Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
Rodrigues DR, Fernandes RK, Balderramas Hde A, Penitenti M, Bachiega TF, Calvi SA, Dias-Melicio LA, Ikoma MR, Soares ÂM
Cytokine 2014 Sep;69(1):102-9
Cytokine 2014 Sep;69(1):102-9
Podosomes of dendritic cells facilitate antigen sampling.
Baranov M, Ter Beest M, Reinieren-Beeren I, Cambi A, Figdor CG, van den Bogaart G
Journal of cell science 2014 Mar 1;127(Pt 5):1052-1064
Journal of cell science 2014 Mar 1;127(Pt 5):1052-1064
Human intestinal epithelial cells respond to β-glucans via Dectin-1 and Syk.
Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I
European journal of immunology 2014 Dec;44(12):3729-40
European journal of immunology 2014 Dec;44(12):3729-40
The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum.
Lefèvre L, Lugo-Villarino G, Meunier E, Valentin A, Olagnier D, Authier H, Duval C, Dardenne C, Bernad J, Lemesre JL, Auwerx J, Neyrolles O, Pipy B, Coste A
Immunity 2013 May 23;38(5):1038-49
Immunity 2013 May 23;38(5):1038-49
Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway.
Ifrim DC, Joosten LA, Kullberg BJ, Jacobs L, Jansen T, Williams DL, Gow NA, van der Meer JW, Netea MG, Quintin J
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 15;190(8):4129-35
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 15;190(8):4129-35
MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
PloS one 2012;7(12):e50589
PloS one 2012;7(12):e50589
Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.
Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ
European journal of immunology 2012 Jun;42(6):1512-22
European journal of immunology 2012 Jun;42(6):1512-22
Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome.
Gringhuis SI, Kaptein TM, Wevers BA, Theelen B, van der Vlist M, Boekhout T, Geijtenbeek TB
Nature immunology 2012 Jan 22;13(3):246-54
Nature immunology 2012 Jan 22;13(3):246-54
Protein kinase Cδ is a critical component of Dectin-1 signaling in primary human monocytes.
Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
Journal of leukocyte biology 2011 Sep;90(3):599-611
Journal of leukocyte biology 2011 Sep;90(3):599-611
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Cabezón R, Sintes J, Llinàs L, Benitez-Ribas D
Immunology letters 2011 Jan 30;134(2):167-73
Immunology letters 2011 Jan 30;134(2):167-73
Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2.
Gringhuis SI, Wevers BA, Kaptein TM, van Capel TM, Theelen B, Boekhout T, de Jong EC, Geijtenbeek TB
PLoS pathogens 2011 Jan 20;7(1):e1001259
PLoS pathogens 2011 Jan 20;7(1):e1001259
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Dectin-1/CLEC7A in Human Dendritic Cells. Dectin-1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
