MAB717

antibody from Novus Biologicals

Targeting: CCL28 CCK1, MEC, SCYA28

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB717

Provider

Novus Biologicals

Product name

Mouse Monoclonal CCL28 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 19, 21, 22, 25, or recombinant rat CCL20 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Brain-to-cervical lymph node signaling after stroke.

Esposito E, Ahn BJ, Shi J, Nakamura Y, Park JH, Mandeville ET, Yu Z, Chan SJ, Desai R, Hayakawa A, Ji X, Lo EH, Hayakawa K
Nature communications 2019 Nov 22;10(1):5306

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Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.

Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30

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Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.

Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):593-603

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Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.

Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):593-603

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CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.

Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61

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CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.

Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1452-61

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB717). The ND₅₀ is typically 15-45 µg/mL.