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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB533-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL28 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL28 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) CCL28 is observed and no cross-reactivity with rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10, 11, 12, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JIL2- Isotype
- IgG
- Antibody clone number
- 134306
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references POL7085 or anti-CCL28 treatment inhibits development of post-paramyxoviral airway disease.
Structure-function analysis of CCL28 in the development of post-viral asthma.
Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo.
Buelow BJ, Rohlfing M, Jung F, Douglas GJ, Grayson MH
Immunity, inflammation and disease 2017 Jun;5(2):98-108
Immunity, inflammation and disease 2017 Jun;5(2):98-108
Structure-function analysis of CCL28 in the development of post-viral asthma.
Thomas MA, Buelow BJ, Nevins AM, Jones SE, Peterson FC, Gundry RL, Grayson MH, Volkman BF
The Journal of biological chemistry 2015 Feb 13;290(7):4528-36
The Journal of biological chemistry 2015 Feb 13;290(7):4528-36
Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
Mosconi E, Rekima A, Seitz-Polski B, Kanda A, Fleury S, Tissandie E, Monteiro R, Dombrowicz DD, Julia V, Glaichenhaus N, Verhasselt V
Mucosal immunology 2010 Sep;3(5):461-74
Mucosal immunology 2010 Sep;3(5):461-74
Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo.
Kutzler MA, Kraynyak KA, Nagle SJ, Parkinson RM, Zharikova D, Chattergoon M, Maguire H, Muthumani K, Ugen K, Weiner DB
Gene therapy 2010 Jan;17(1):72-82
Gene therapy 2010 Jan;17(1):72-82
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL28 Monoclonal Antibody (Catalog # MAB533). The ND50 is typically 2.5-5.0 µg/mL.
