PA5-120413

antibody from Invitrogen Antibodies

Targeting: FREM2 DKFZp686J0811

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120413

Provider

Invitrogen Antibodies

Product name

FREM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: Jurkat, HepG2, 293T. The target is usually found in the following locations: Cell membrane, Extracellular side, Single-pass type I membrane protein.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.43 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FREM2 in various cell lines using a FREM2 Polyclonal antibody (Product # PA5-120413) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 150s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of FREM2 in NIH/3T3 cells using a FREM2 Polyclonal antibody (Product # PA5-120413). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of FREM2 in NIH/3T3 cells using a FREM2 Polyclonal antibody (Product # PA5-120413). Blue: DAPI for nuclear staining.