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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 14270-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITPKA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LLSDSESRS RGNVQLEAGE DVGQKNHWQK IRTMVNLPVI SPFKKRYAWV QLAGHTGSFK AAGTSGLILK RCSEPERYCL ARLMADALRG CVPAFHGVVE RDGESYLQLQ DLLDGFDGPC VLDCKMGVRT YLEEELTKAR ERPKLRKDMY KKMLAVDPEA PTEEEHAQRA VTKPRYMQWR EGISSSTTLG FRIEGIKKAD GSCSTDFKTT RSREQVLRVF EEFVQGDEEV LRRYLNRLQQ IRDTLEVSEF FRRHEVIGSS LLFVHDHCHR AGVWLIDFGK TTPLPDGQIL DHRRPWEEGN REDGYLLGLD NLIGILASLA ER (141-461 aa encoded by BC026331)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14270-1-AP (ITPKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14270-1-AP (ITPKA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ITPKA antibody 14270-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14270-1-AP ( ITPKA Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14270-1-AP ( ITPKA Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ITPKA (IP:14270-1-AP, 4ug; Detection:14270-1-AP 1:400) with mouse brain tissue lysate 4000ug.
