Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AMR-062-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRGPRE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human U-87 MG glioblastoma: - 1. Anti-MRGPRE Antibody (#AMR-062), (1:400). 2. Anti-MRGPRE Antibody , preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human U-87 MG glioblastoma: - 1. Anti-MRGPRE Antibody (#AMR-062), (1:400). 2. Anti-MRGPRE Antibody , preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 4), mouse (lanes 2 and 5) and rat (lanes 3 and 6)brain membranes: - 1-3. Anti-MRGPRE Antibody (#AMR-062), (1:400).4-6. Anti-MRGPRE Antibody , preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of MRGE in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MRGPRE Antibody (#AMR-062), (1:200), followed by goat- Anti-rabbit-AlexaFluor-488. MRGE staining (green) in hippocampal dentate gyrus is detected in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).