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- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB1059P-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NKG2A/CD159a PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 131411
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Altered NKp30, NKp46, NKG2D, and DNAM-1 Expression on Circulating NK Cells Is Associated with Tumor Progression in Human Gastric Cancer.
Analysis of NK cell clones obtained using interleukin-2 and gene-modified K562 cells revealed the ability of "senescent" NK cells to lose CD57 expression and start expressing NKG2A.
Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-β and Engagement of CD85j.
Eomesodermin and T-bet mark developmentally distinct human natural killer cells.
Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-β-dependent emperipolesis in HBV cirrhotic patients.
Alteration of Natural Killer cell phenotype and function in obese individuals.
CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients.
NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
Up-regulation of activating and inhibitory NKG2 receptors in allogeneic and autologous hematopoietic stem cell grafts.
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Overexpression of CD85j in TNBC patients inhibits Cetuximab-mediated NK-cell ADCC but can be restored with CD85j functional blockade.
Long-term effect on natural killer cells by interferon-α therapy on the outcomes of HCV infection.
Intracellular poly(I:C) initiated gastric adenocarcinoma cell apoptosis and subsequently ameliorated NK cell functions.
Natural killer cells are characterized by the concomitantly increased interferon-γ and cytotoxicity in acute resolved hepatitis B patients.
A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
Phenotype markers and cytokine intracellular production by CD8+ gammadelta T lymphocytes do not support a regulatory T profile in Behçet's disease patients and healthy controls.
Isolation and characterization of CD8+ regulatory T cells in multiple sclerosis.
Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil.
Functional impairment in circulating and intrahepatic NK cells and relative mechanism in hepatocellular carcinoma patients.
Han B, Mao FY, Zhao YL, Lv YP, Teng YS, Duan M, Chen W, Cheng P, Wang TT, Liang ZY, Zhang JY, Liu YG, Guo G, Zou QM, Zhuang Y, Peng LS
Journal of immunology research 2018;2018:6248590
Journal of immunology research 2018;2018:6248590
Analysis of NK cell clones obtained using interleukin-2 and gene-modified K562 cells revealed the ability of "senescent" NK cells to lose CD57 expression and start expressing NKG2A.
Streltsova MA, Erokhina SA, Kanevskiy LM, Lee DA, Telford WG, Sapozhnikov AM, Kovalenko EI
PloS one 2018;13(12):e0208469
PloS one 2018;13(12):e0208469
Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-β and Engagement of CD85j.
Nuñez SY, Ziblat A, Secchiari F, Torres NI, Sierra JM, Raffo Iraolagoitia XL, Araya RE, Domaica CI, Fuertes MB, Zwirner NW
Journal of immunology (Baltimore, Md. : 1950) 2018 Feb 1;200(3):1008-1015
Journal of immunology (Baltimore, Md. : 1950) 2018 Feb 1;200(3):1008-1015
Eomesodermin and T-bet mark developmentally distinct human natural killer cells.
Collins A, Rothman N, Liu K, Reiner SL
JCI insight 2017 Mar 9;2(5):e90063
JCI insight 2017 Mar 9;2(5):e90063
Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-β-dependent emperipolesis in HBV cirrhotic patients.
Shi J, Zhao J, Zhang X, Cheng Y, Hu J, Li Y, Zhao X, Shang Q, Sun Y, Tu B, Shi L, Gao B, Wang FS, Zhang Z
Scientific reports 2017 Mar 14;7:44544
Scientific reports 2017 Mar 14;7:44544
Alteration of Natural Killer cell phenotype and function in obese individuals.
Viel S, Besson L, Charrier E, Marçais A, Disse E, Bienvenu J, Walzer T, Dumontet C
Clinical immunology (Orlando, Fla.) 2017 Apr;177:12-17
Clinical immunology (Orlando, Fla.) 2017 Apr;177:12-17
CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients.
Guo C, Shen X, Fu B, Liu Y, Chen Y, Ni F, Ye Y, Sun R, Li J, Tian Z, Wei H
Scientific reports 2016 May 13;6:25567
Scientific reports 2016 May 13;6:25567
NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
PloS one 2015;10(8):e0134568
PloS one 2015;10(8):e0134568
Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hübscher D, Walter L, Streckfuss-Bömeke K, Guan K, Dressel R
PloS one 2015;10(5):e0125544
PloS one 2015;10(5):e0125544
Up-regulation of activating and inhibitory NKG2 receptors in allogeneic and autologous hematopoietic stem cell grafts.
Picardi A, Mengarelli A, Marino M, Gallo E, Benevolo M, Pescarmona E, Cocco R, Fraioli R, Tremante E, Petti MC, De Fabritiis P, Giacomini P
Journal of experimental & clinical cancer research : CR 2015 Sep 11;34:98
Journal of experimental & clinical cancer research : CR 2015 Sep 11;34:98
Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Spallanzani RG, Torres NI, Avila DE, Ziblat A, Iraolagoitia XL, Rossi LE, Domaica CI, Fuertes MB, Rabinovich GA, Zwirner NW
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2141-8
Overexpression of CD85j in TNBC patients inhibits Cetuximab-mediated NK-cell ADCC but can be restored with CD85j functional blockade.
Roberti MP, Juliá EP, Rocca YS, Amat M, Bravo AI, Loza J, Coló F, Loza CM, Fabiano V, Maino M, Podhorzer A, Fainboim L, Barrio MM, Mordoh J, Levy EM
European journal of immunology 2015 May;45(5):1560-9
European journal of immunology 2015 May;45(5):1560-9
Long-term effect on natural killer cells by interferon-α therapy on the outcomes of HCV infection.
Hu X, Jiang Y, Li X, Gao Y, Guo X, Chi X, Yan H, Feng J, Zhong J, Sun B, Shao X, Xiao W, Pan Y, Niu J
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 May;34(5):366-75
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 May;34(5):366-75
Intracellular poly(I:C) initiated gastric adenocarcinoma cell apoptosis and subsequently ameliorated NK cell functions.
Qu J, Hou Z, Han Q, Jiang W, Zhang C, Tian Z, Zhang J
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 Jan;34(1):52-9
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 Jan;34(1):52-9
Natural killer cells are characterized by the concomitantly increased interferon-γ and cytotoxicity in acute resolved hepatitis B patients.
Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang FS
PloS one 2012;7(11):e49135
PloS one 2012;7(11):e49135
A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
Ogbomo H, Michaelis M, Altenbrandt B, Doerr HW, Cinatl J Jr
Biochemical pharmacology 2010 Jan 15;79(2):188-97
Biochemical pharmacology 2010 Jan 15;79(2):188-97
Phenotype markers and cytokine intracellular production by CD8+ gammadelta T lymphocytes do not support a regulatory T profile in Behçet's disease patients and healthy controls.
Clemente A, Cambra A, Munoz-Saá I, Crespí C, Pallarés L, Juan A, Matamoros N, Julià MR
Immunology letters 2010 Apr 8;129(2):57-63
Immunology letters 2010 Apr 8;129(2):57-63
Isolation and characterization of CD8+ regulatory T cells in multiple sclerosis.
Correale J, Villa A
Journal of neuroimmunology 2008 Mar;195(1-2):121-34
Journal of neuroimmunology 2008 Mar;195(1-2):121-34
Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil.
Teixeira-Carvalho A, Fujiwara RT, Stemmy EJ, Olive D, Damsker JM, Loukas A, Corrêa-Oliveira R, Constant SL, Bethony JM
Infection and immunity 2008 Dec;76(12):5810-6
Infection and immunity 2008 Dec;76(12):5810-6
Functional impairment in circulating and intrahepatic NK cells and relative mechanism in hepatocellular carcinoma patients.
Cai L, Zhang Z, Zhou L, Wang H, Fu J, Zhang S, Shi M, Zhang H, Yang Y, Wu H, Tien P, Wang FS
Clinical immunology (Orlando, Fla.) 2008 Dec;129(3):428-37
Clinical immunology (Orlando, Fla.) 2008 Dec;129(3):428-37
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2A/CD159a PE-conjugated Monoclonal Antibody (Catalog # FAB1059P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
