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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF7869 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SNAP29 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human SNAP29 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O95721- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor.
Zhu Q, Yamakuchi M, Lowenstein CJ
PloS one 2015;10(8):e0118737
PloS one 2015;10(8):e0118737
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SNAP29 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SNAP29 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SNAP29 in K562 Human Cell Line. SNAP29 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Sheep Anti-Human SNAP29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7869) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi granules in cytoplasm (STX6 staining shown in green, lower panel). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
