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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF2030 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal VASA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human VASA in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice.
Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance.
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.
Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells.
PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation.
Quantitative Proteomic Profiling of the Human Ovary from Early to Mid-Gestation Reveals Protein Expression Dynamics of Oogenesis and Folliculogenesis.
Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23.
Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility.
Characterization of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.
Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling.
Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.
Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies.
Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?
Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells.
Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
Complete meiosis from human induced pluripotent stem cells.
A novel approach for the derivation of putative primordial germ cells and sertoli cells from human embryonic stem cells.
Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
Generation of pluripotent stem cells from adult human testis.
Isolation of primordial germ cells from differentiating human embryonic stem cells.
Bagheri-Fam S, Chen H, Wilson S, Ayers K, Hughes J, Sloan-Bena F, Calvel P, Robevska G, Puisac B, Kusz-Zamelczyk K, Gimelli S, Spik A, Jaruzelska J, Warenik-Szymankiewicz A, Faradz S, Nef S, Pié J, Thomas P, Sinclair A, Wilhelm D
PloS one 2020;15(1):e0227411
PloS one 2020;15(1):e0227411
Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance.
Stringer JM, Forster SC, Qu Z, Prokopuk L, O'Bryan MK, Gardner DK, White SJ, Adelson D, Western PS
BMC biology 2018 Sep 20;16(1):104
BMC biology 2018 Sep 20;16(1):104
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.
Hermann BP, Cheng K, Singh A, Roa-De La Cruz L, Mutoji KN, Chen IC, Gildersleeve H, Lehle JD, Mayo M, Westernströer B, Law NC, Oatley MJ, Velte EK, Niedenberger BA, Fritze D, Silber S, Geyer CB, Oatley JM, McCarrey JR
Cell reports 2018 Nov 6;25(6):1650-1667.e8
Cell reports 2018 Nov 6;25(6):1650-1667.e8
Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells.
Vértesy Á, Arindrarto W, Roost MS, Reinius B, Torrens-Juaneda V, Bialecka M, Moustakas I, Ariyurek Y, Kuijk E, Mei H, Sandberg R, van Oudenaarden A, Chuva de Sousa Lopes SM
Nature communications 2018 May 14;9(1):1873
Nature communications 2018 May 14;9(1):1873
PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation.
Gell JJ, Zhao J, Chen D, Hunt TJ, Clark AT
Stem cell research 2018 Mar;27:46-56
Stem cell research 2018 Mar;27:46-56
Quantitative Proteomic Profiling of the Human Ovary from Early to Mid-Gestation Reveals Protein Expression Dynamics of Oogenesis and Folliculogenesis.
Bothun AM, Gao Y, Takai Y, Ishihara O, Seki H, Karger B, Tilly JL, Woods DC
Stem cells and development 2018 Jun 1;27(11):723-735
Stem cells and development 2018 Jun 1;27(11):723-735
Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23.
Clark AT, Gkountela S, Chen D, Liu W, Sosa E, Sukhwani M, Hennebold JD, Orwig KE
Stem cell reports 2017 Jul 11;9(1):329-341
Stem cell reports 2017 Jul 11;9(1):329-341
Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility.
Wang N, Satirapod C, Ohguchi Y, Park ES, Woods DC, Tilly JL
Scientific reports 2017 Aug 30;7(1):10011
Scientific reports 2017 Aug 30;7(1):10011
Characterization of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.
Eguizabal C, Herrera L, De Oñate L, Montserrat N, Hajkova P, Izpisua Belmonte JC
Stem cells (Dayton, Ohio) 2016 Sep;34(9):2418-28
Stem cells (Dayton, Ohio) 2016 Sep;34(9):2418-28
Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling.
Hu YC, Nicholls PK, Soh YQ, Daniele JR, Junker JP, van Oudenaarden A, Page DC
PLoS genetics 2015 Mar;11(3):e1005019
PLoS genetics 2015 Mar;11(3):e1005019
Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.
Heeren AM, van Iperen L, Klootwijk DB, de Melo Bernardo A, Roost MS, Gomes Fernandes MM, Louwe LA, Hilders CG, Helmerhorst FM, van der Westerlaken LA, Chuva de Sousa Lopes SM
BMC developmental biology 2015 Jan 21;15:4
BMC developmental biology 2015 Jan 21;15:4
Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
Lim JJ, Shim MS, Lee JE, Lee DR
PloS one 2014;9(4):e90454
PloS one 2014;9(4):e90454
Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies.
Dominguez AA, Chiang HR, Sukhwani M, Orwig KE, Reijo Pera RA
Scientific reports 2014 Sep 22;4:6432
Scientific reports 2014 Sep 22;4:6432
Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes SM, De Sutter P
Stem cells and development 2013 Dec 1;22(23):3141-55
Stem cells and development 2013 Dec 1;22(23):3141-55
Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?
Bhartiya D, Sriraman K, Gunjal P, Modak H
Journal of ovarian research 2012 Nov 7;5(1):32
Journal of ovarian research 2012 Nov 7;5(1):32
Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells.
Geens M, Sermon KD, Van de Velde H, Tournaye H
Journal of assisted reproduction and genetics 2011 May;28(5):471-80
Journal of assisted reproduction and genetics 2011 May;28(5):471-80
Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL
Stem cells and development 2011 Jun;20(6):1079-88
Stem cells and development 2011 Jun;20(6):1079-88
Complete meiosis from human induced pluripotent stem cells.
Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Izpisua Belmonte JC
Stem cells (Dayton, Ohio) 2011 Aug;29(8):1186-95
Stem cells (Dayton, Ohio) 2011 Aug;29(8):1186-95
A novel approach for the derivation of putative primordial germ cells and sertoli cells from human embryonic stem cells.
Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
Stem cells (Dayton, Ohio) 2009 Jan;27(1):68-77
Stem cells (Dayton, Ohio) 2009 Jan;27(1):68-77
Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL
Stem cells (Dayton, Ohio) 2008 Nov;26(11):2768-76
Stem cells (Dayton, Ohio) 2008 Nov;26(11):2768-76
Generation of pluripotent stem cells from adult human testis.
Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Bühring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
Nature 2008 Nov 20;456(7220):344-9
Nature 2008 Nov 20;456(7220):344-9
Isolation of primordial germ cells from differentiating human embryonic stem cells.
Tilgner K, Atkinson SP, Golebiewska A, Stojkovic M, Lako M, Armstrong L
Stem cells (Dayton, Ohio) 2008 Dec;26(12):3075-85
Stem cells (Dayton, Ohio) 2008 Dec;26(12):3075-85
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human VASA by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- VASA in Human Testis. VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
