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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2479 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2479, RRID:AB_1574284
- Product name
- DDX4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DDX4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia.
VASA is a specific marker for both normal and malignant human germ cells.
The human VASA gene is specifically expressed in the germ cell lineage.
Guo X, Gui YT, Tang AF, Lu LH, Gao X, Cai ZM
Asian journal of andrology 2007 May;9(3):339-44
Asian journal of andrology 2007 May;9(3):339-44
VASA is a specific marker for both normal and malignant human germ cells.
Zeeman AM, Stoop H, Boter M, Gillis AJ, Castrillon DH, Oosterhuis JW, Looijenga LH
Laboratory investigation; a journal of technical methods and pathology 2002 Feb;82(2):159-66
Laboratory investigation; a journal of technical methods and pathology 2002 Feb;82(2):159-66
The human VASA gene is specifically expressed in the germ cell lineage.
Castrillon DH, Quade BJ, Wang TY, Quigley C, Crum CP
Proceedings of the National Academy of Sciences of the United States of America 2000 Aug 15;97(17):9585-90
Proceedings of the National Academy of Sciences of the United States of America 2000 Aug 15;97(17):9585-90
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of DDX4 polyclonal antibody (Cat # PAB2479) in K-562 cell line lysates (35 ug/lane). DDX4 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 polyclonal antibody (Cat # PAB2479) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
