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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF2675 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NTH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human NTH1 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P78549- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Transcriptional regulation of the base excision repair pathway by BRCA1.
Saha T, Rih JK, Roy R, Ballal R, Rosen EM
The Journal of biological chemistry 2010 Jun 18;285(25):19092-105
The Journal of biological chemistry 2010 Jun 18;285(25):19092-105
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human NTH1 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human NTH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NTH1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
