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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-57754 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UGDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IDMNDYQRRR FASRIIDSLF NTVTDKKIAI LGFAFKKDTG DTRESSSIYI SKYLMDEGAH LHIYDPKVPR EQIVVDLSHP GVSEDDQVSR LV
- Concentration
- 0.1 mg/mL
Submitted references UDP-glucose dehydrogenase (UGDH) activity is suppressed by peroxide and promoted by PDGF in fibroblast-like synoviocytes: Evidence of a redox control mechanism.
Chandrasekaran R, Mathieu C, Sheth R, Cheng AP, Fong D, McCormack R, El-Gabalawy H, Alishetty S, Paige M, Hoemann CD
PloS one 2022;17(9):e0274420
PloS one 2022;17(9):e0274420
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UGDH in control (vector only transfected HEK293T lysate) and UGDH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a UGDH Polyclonal Antibody (Product # PA5-57754).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UGDH in human prostate using UGDH Polyclonal Antibody (Product # PA5-57754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UGDH in human colon using UGDH Polyclonal Antibody (Product # PA5-57754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UGDH in human liver using UGDH Polyclonal Antibody (Product # PA5-57754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UGDH in human kidney using UGDH Polyclonal Antibody (Product # PA5-57754).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UGDH in human liver using an UGDH Polyclonal Antibody (Product # PA5-57754) shows strong cytoplasmic positivity in hepatocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- S2 Fig UGDH protein showed detectable nuclear staining in selected cells. (A-D) Phase contrast and (E-H) matching epifluorescent images of UGDH immunostain and (I-L) DAPI-counterstained fluorescent nuclei (I-L) of FLS (P4) extracted from a synovial biopsy from a 23-year old male donor and cultured under different cytokine conditions as indicated, fixed in acetone, and immunostained for UGDH with red substrate detection which is fluorescent. Symbols: small white arrows: cytosolic UGDH immunostain; open arrows: detectable nuclear UGDH immunostain. Scale bars: 20 mum. (TIF) Click here for additional data file.
