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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 91319 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H4K5ac/K8ac Recombinant Mouse Monoclonal Antibody, AbFlex®
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) tested by Western blot 20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H4K5ac/K8ac antibody at 2 µg/mL. Lane 1: nuclear extract from untreated cells. Lane 2: nuclear extract from cells treated with sodium butyrate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) specificity tested by ELISA 10, 2 or 0.4 pmole of various biotinylated Histone H4 peptides were captured in duplicate wells of a Streptavidin coated plates. 0.5 µg/mL of Histone H4K5ac/K8ac antibody was added and detected using secondary anti-mouse HRP antibody. Histone H4K5ac/K8ac antibody recognizes peptide containing both K5ac and K8ac. The presence of additional K12 and K16 acetylation does not affect detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex Histone H4K5ac/K8ac recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 µg of HeLa cell chromatin and 4 µg of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 6.7 million sequence tags were mapped to identify Histone H4K5ac/K8ac binding sites on chromosome 1.
