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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-804-674-L001 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-674-L001, RRID:AB_2050478
- Product name
- [K20-monomethyl]Histone H4 monoclonal antibody (5E10-D8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5E10-D8
- Vial size
- 1 ml
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Peptide dot blot: 50pmol or 10pmol of the indicated peptides corresponding to aa 12-31 of histone H4 and containing either unmethylated, or mono-, di-, or trimethylated lysine 20 were spotted onto a PVDF membrane. After blocking for 60 min. in 3% NFDM in PBS-T, the membrane was incubated for 60 min. at RT with anti-H4K20me1 MAb (5E10-D8) (Prod. No. ALX-804-674), dilution 1:5, and processed for Western blotting.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western Blot against HeLa histones (approx. 2µg loaded per lane) withÊanti-H4K20me1ÊMAb (5E10-D8) (Prod. No. ALX-804-674) at the indicated dilutions, and, as control, a high concentration Fibercell supernatant of anti-H3K9me3 MAb (6F12-H4) (Prod. No. ALX-804-673)Êdilution 1:500, 90 sec exposure.
