HPA005939

antibody from Atlas Antibodies

Targeting: NIN

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA005939

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005939, RRID:AB_2667474

Product name

Anti-NIN

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EERLTQMRNEYERQCRVLQDQVDELQSELEEYRAQ
GRVLRLPLKNSPSEEVEANSGGIEPEHGLGSEECN
PLNMSIEAELVIEQMKEQHHRDICCLRLELEDKVR
HYEKQLDETVVSCKKAQENMKQRHENETHTL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & centrosome.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human appendix and pancreas tissues using Anti-NIN antibody. Corresponding NIN RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows distinct perinuclear positivity in lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN