AF8555

antibody from R&D Systems

Targeting: A4GNT alpha4GnT

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF8555

Provider

R&D Systems

Product name

Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human alpha-1,4-N-Acetylglucosaminyltransferase in direct ELISAs and Western blots.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

Q9UNA3


Isotype

IgG

Vial size

100 uL

Storage

12 months from date of receipt, -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 °C under sterile conditions after opening.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, KATO-III human gastric carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.