PA5-42916

antibody from Invitrogen Antibodies

Targeting: CORIN ATC2, CRN, Lrp4, PRSC, TMPRSS10

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-42916

Provider

Invitrogen Antibodies

Product name

CORIN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: HPRYSRAVVD YDISIVELSE DISETGYVRP VCLPNPEQWL EPDTYCYITG

Concentration

1 mg/mL

References

Submitted references

Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes.

Bae IS, Kim SH
International journal of molecular sciences 2019 Dec 5;20(24)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human HepG2 cell lysate using an anti-CORIN polyclonal antibody (Product # PA5-42916).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 The expression of corin and ANP was increased in DHA-induced adipocytes. 3T3-L1 cells were exposed to DHA (100 muM) for 2 d in the presence of the differentiation medium. ( A - C ) The expression of corin and ANP in DHA-induced adipocytes was analyzed by qRT-PCR and Western blotting. The basal delta-Ct levels for tested genes are presented as Supplementary Table S2 . ** p < 0.01. ( D , E ) The expression of corin and ANP was measured in the GPR120 deficient adipocytes treated with DHA by qRT-PCR and Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001. ( F ) 3T3-L1 cells were treated with 1 muM TUG-891, a potent GPR120 agonist for 24 h. The corin and ANP expression levels were analyzed by Western blotting. ( G ) The concentration of ANP was measured in the media derived from the DHA-induced adipocytes using ELISA. ** p < 0.01. The data are shown as the means +- standard deviations from three or more independent experiments.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 The expression of corin and ANP was increased in beige-like adipocytes induced by T3 and rosiglitazone. ( A - C ) The expression of corin and ANP in white adipocytes and beige adipocytes was analyzed by qRT-PCR and Western blotting. The basal delta-Ct levels for tested genes are presented as Supplementary Table S4 . ** p < 0.01, *** p < 0.001. ( D ) The concentration of ANP was measured in the media of adipocytes by ELISA. ** p < 0.01. The representative data are presented as the mean +- standard deviation of three independent experiments.