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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-57279 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TFF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SSAEEYVGLS ANQCAVPAKD RVDCGYPHVT PKECNNRGCC FDSRIPGVPW CFKPLQEAEC T
- Concentration
- 0.1 mg/mL
Submitted references Building a Thick Mucus Hydrogel Layer to Improve the Physiological Relevance of In Vitro Primary Colonic Epithelial Models.
Wang Y, Kim R, Sims CE, Allbritton NL
Cellular and molecular gastroenterology and hepatology 2019;8(4):653-655.e5
Cellular and molecular gastroenterology and hepatology 2019;8(4):653-655.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TFF3 in human cell line MCF7 using a TFF3 Polyclonal Antibody (Product # PA5-57279) shows localization to nucleus and nucleoli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TFF3 in human colon and skeletal muscle tissues using TFF3 Polyclonal Antibody (Product # PA5-57279). Corresponding TFF3 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TFF3 in human small intestine using TFF3 Polyclonal Antibody (Product # PA5-57279) shows strong positivity in mucus in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TFF3 in human tonsil using TFF3 Polyclonal Antibody (Product # PA5-57279) shows no positivity in lymphoid cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TFF3 in human colon using TFF3 Polyclonal Antibody (Product # PA5-57279) shows moderate to strong positivity in mucus in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TFF3 in human skeletal muscle using TFF3 Polyclonal Antibody (Product # PA5-57279) shows no positivity in myocytes as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 VIP-assisted ALI culture generates physiologic mucus layer above intestinal epithelial cells. ( A ) Culture schematic. ( B ) Dose-dependent water secretion of VIP at 24 hours. ( C ) Immunofluorescence of monolayers differentiated under submerged and ALI conditions in the presence of VIP. Green: anti-Muc2; blue: Hoechst 33342. ( D ) Removal of hydrated mucus with forceps. ( E ) Side-view confocal micrograph showing tissues with mucus separating bacteria from epithelial cells at 0 and 6 days, respectively. Green: green fluorescent protein (GFP)-expressing Escherichia coli ; blue: Hoechst 33342. ( F ) Plot of mucus thickness vs duration of ALI. ( G ) Representative side-view confocal micrograph showing 1 mum red fluorescent beads separated from epithelial cells by mucus. ( H ) Immunofluorescence staining of paraffin-embedded, sectioned monolayers. DM, differentiation medium.
