PB9486

antibody from Boster Biological Technology

Targeting: CD46 MCP, MGC26544, MIC10, TLX, TRA2.10

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PB9486

Provider

Boster Biological Technology

Product name

Anti-CD46 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CD46 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CD46 information: Molecular Weight: 43747 MW; Subcellular Localization: Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane ; Single- pass type I membrane protein . Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide; Tissue Specificity: Expressed by all cells except erythrocytes.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of H-PBMC cells using anti- CD46 antibody (PB9486). Overlay histogram showing H-PBMC cells stained with PB9486 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CD46 Antibody (PB9486,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image