AF3430

antibody from Novus Biologicals

Targeting: PTPRS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF3430

Provider

Novus Biologicals

Product name

Goat Polyclonal PTP sigma/PTPRS Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects the endogenous cleaved extracellular domain of human PTP sigma/PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPR beta , PTPR delta , PTPRµ, or PTPR kappa is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Roles of Collagen XXV and Its Putative Receptors PTPσ/δ in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder.

Munezane H, Oizumi H, Wakabayashi T, Nishio S, Hirasawa T, Sato T, Harada A, Yoshida T, Eguchi T, Yamanashi Y, Hashimoto T, Iwatsubo T
Cell reports 2019 Dec 24;29(13):4362-4376.e6

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Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.

Dyck S, Kataria H, Alizadeh A, Santhosh KT, Lang B, Silver J, Karimi-Abdolrezaee S
Journal of neuroinflammation 2018 Mar 20;15(1):90

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Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injury.

Lang BT, Cregg JM, DePaul MA, Tran AP, Xu K, Dyck SM, Madalena KM, Brown BP, Weng YL, Li S, Karimi-Abdolrezaee S, Busch SA, Shen Y, Silver J
Nature 2015 Feb 19;518(7539):404-8

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Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells.

Bunin A, Sisirak V, Ghosh HS, Grajkowska LT, Hou ZE, Miron M, Yang C, Ceribelli M, Uetani N, Chaperot L, Plumas J, Hendriks W, Tremblay ML, Häcker H, Staudt LM, Green PH, Bhagat G, Reizis B
Immunity 2015 Aug 18;43(2):277-88

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PTP sigma/PTPRS by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human PTPRµ, PTPR kappa , LAR, PTPR sigma , PTPR delta , and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.