13389-1-AP

antibody from Invitrogen Antibodies

Targeting: PFKP PFK-C, PFKF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13389-1-AP

Provider

Invitrogen Antibodies

Product name

PFKP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GIADGHRML AIYDGFDGFA KGQIKEIGWT DVGGWTGQGG SILGTKRVLP GKYLEEIATQ MRTHSINALL IIGGFEAYLG LLELSAAREK HEEFCVPMVM VPATVSNNVP GSDFSIGADT ALNTITDTCD RIKQSASGTK RRVFIIETMG GYCGYLANMG GLAAGADAAY IFEEPFDIRD LQSNVEHLTE KMKTTIQRGL VLRNESCSEN YTTDFIYQLY SEEGKGVFDC RKNVLGHMQQ GGAPSPFDRN FGTKISARAM EWITAKLKEA RGRGKKFTTD DSICVLGISK RNVIFQPVAE LKKQTDFEHR IPKEQWWLKL RPLMKILAKY KASYDVSDSG QLEHVQPWSV (436-784 aa encoded by BC029138)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BxPC-3 cells using 13389-1-AP (PFKP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 13389-1-AP ( PFKP Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 13389-1-AP ( PFKP Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PFKP (IP:13389-1-AP, 4 ug; Detection:13389-1-AP 1:1000) with Jurkat cells lysate 3400 ug.