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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00003263-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003263-M01, RRID:AB_539660
- Product name
- Mouse Monoclonal Hemopexin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HPX - hemopexin
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Analysis of HPX over-expressed 293 cell line, cotransfected with HPX Validated Chimera RNAi ( Cat # H00003263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HPX monoclonal antibody (M01), clone 3A9-1A9 (Cat # H00003263-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Analysis of HPX expression in transfected 293T cell line by HPX monoclonal antibody (M01), clone 3A9-1A9.Lane 1: HPX transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - HPX monoclonal antibody (M01), clone 3A9-1A9. Analysis of HPX expression in human liver.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Hemopexin Antibody (3A9-1A9) [H00003263-M01] - Detection limit for recombinant GST tagged HPX is 0.3 ng/ml as a capture antibody.
