LS-B14321

antibody from LSBio

Targeting: FERMT2 KIND2, mig-2, PLEKHC1, UNC112B

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B14321

Provider

LSBio

Product name

IHC-plus™ KIND2 / FERMT2 Antibody (clone 25A4) LS-B14321

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

25A4

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation(IP) of FERMT2 by using monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FERMT2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of FERMT2 by using monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FERMT2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Ovary tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Ovary tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50).