Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- CF500505 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FERMT2 Monoclonal Antibody (OTI14A11), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI14A11
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT. Positive lysates LY416377 (100 µg) and LC416377 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT. Positive lysates LY416377 (100 µg) and LC416377 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and FERMT2-Knockout HeLa cells (KO, Cat# LC812552) were separated by SDS-PAGE and immunoblotted with anti-FERMT2 monoclonal antibody TA500505(1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from a mouse cell line and a mouse tissue by using anti-FERMT2 monoclonal antibody. (1:200)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-FERMT2 mouse monoclonal antibody (TA500505) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500505).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500505)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.