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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX106854 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX106854, RRID:AB_1952473
- Product name
- U2AF35 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: HepG2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)12% SDS PAGEGTX106854 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using U2AF35(GTX106854) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of U2AF35 protein from 293T whole cell extracts using 5 £gg of U2AF35 antibody (GTX106854).Western blot analysis was performed using U2AF35 antibody (GTX106854).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- U2AF35 antibody detects U2AF35 protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. U2AF35 antibody (GTX106854) diluted at 1:1000.
